Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany.
Identifieur interne : 000025 ( Main/Exploration ); précédent : 000024; suivant : 000026Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany.
Auteurs : G. Moré [Allemagne] ; A. Maksimov [Allemagne] ; F J Conraths [Allemagne] ; G. Schares [Allemagne]Source :
- Veterinary parasitology [ 1873-2550 ] ; 2016.
English descriptors
- KwdEn :
- Animals, Coinfection, Foxes (parasitology), Germany (epidemiology), Intestine, Small (parasitology), Molecular Sequence Data, Oocysts, RNA, Ribosomal, 18S (genetics), Raccoon Dogs (parasitology), Sarcocystis (classification), Sarcocystis (genetics), Sarcocystis (physiology), Sarcocystosis (epidemiology), Sarcocystosis (parasitology), Sarcocystosis (veterinary), Sequence Homology, Nucleic Acid.
- MESH :
- chemical , genetics : RNA, Ribosomal, 18S.
- classification : Sarcocystis.
- epidemiology : Germany, Sarcocystosis.
- genetics : Sarcocystis.
- parasitology : Foxes, Intestine, Small, Raccoon Dogs, Sarcocystosis.
- physiology : Sarcocystis.
- veterinary : Sarcocystosis.
- Animals, Coinfection, Molecular Sequence Data, Oocysts, Sequence Homology, Nucleic Acid.
Abstract
More than 200 Sarcocystis spp. have been named and most of them appear to be involved in a particular predator-prey cycle. Among canids, the European fox (Vulpes vulpes) and the raccoon dog (Nyctereutes procyonoides) are widely distributed in Europe and probably play an important role as definitive hosts in the epidemiology of Sarcocystis spp. infections. A total of 50 small intestines from foxes and 38 from raccoon dogs were sampled in the Federal State of Brandenburg, Germany. Mucosal scrapings were collected and analyzed by sugar flotation and when oocysts or sporocysts were detected, an overnight sedimentation was performed and DNA extracted with a commercial kit. A PCR was conducted using primers targeting a fragment of the 18S rRNA gene (with a size of approximately 850 bp) and the amplicons were purified and sequenced. Samples with an inconclusive sequencing were cloned into plasmids and ≥ 3 plasmids from each amplicon were sequenced. Sarcocystis spp. oocysts/sporocysts were detected in 38% (19/50) of fox and 52.6% (20/38) of raccoon dog samples. Sequencing analysis of amplicons from oocyst DNA revealed mixed infections in 9 fox and 5 raccoon dog samples. In the fox samples, the most often identified Sarcocystis spp. were S. tenella or S. capracanis (10.0%); S. miescheriana (8.0%) and S. gracilis (8.0%) followed by Sarcocystis spp., which use birds as intermediate hosts (6.0%), and S. capreolicanis (4.0%). In the raccoon dog samples, sequences with a ≥99% identity with the following species were detected: S. miescheriana (18.4%), S. gracilis (13.1%), Sarcocystis spp. using birds as IH (10.5%), S. tenella or S.capracanis (2.6%) and S. capreolicanis (2.6%). The estimated prevalence of Sarcocystis spp. infections determined using mucosal scrapings was higher than in related studies performed by analyzing faecal samples. The methodology of 18S rRNA gene amplification, cloning and sequencing is suitable to identify mixed infections with Sarcocystis spp. and to gather information on potential definitive hosts of these parasite species.
DOI: 10.1016/j.vetpar.2016.02.011
PubMed: 26995715
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000024
- to stream PubMed, to step Curation: 000024
- to stream PubMed, to step Checkpoint: 000024
- to stream Ncbi, to step Merge: 000452
- to stream Ncbi, to step Curation: 000452
- to stream Ncbi, to step Checkpoint: 000452
- to stream Main, to step Merge: 000024
- to stream Main, to step Curation: 000025
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany.</title>
<author><name sortKey="More, G" sort="More, G" uniqKey="More G" first="G" last="Moré">G. Moré</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems, Germany. Electronic address: gastonmore@fcv.unlp.edu.ar.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems</wicri:regionArea>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Maksimov, A" sort="Maksimov, A" uniqKey="Maksimov A" first="A" last="Maksimov">A. Maksimov</name>
<affiliation wicri:level="1"><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems</wicri:regionArea>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Conraths, F J" sort="Conraths, F J" uniqKey="Conraths F" first="F J" last="Conraths">F J Conraths</name>
<affiliation wicri:level="1"><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems</wicri:regionArea>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Schares, G" sort="Schares, G" uniqKey="Schares G" first="G" last="Schares">G. Schares</name>
<affiliation wicri:level="1"><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems</wicri:regionArea>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:26995715</idno>
<idno type="pmid">26995715</idno>
<idno type="doi">10.1016/j.vetpar.2016.02.011</idno>
<idno type="wicri:Area/PubMed/Corpus">000024</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000024</idno>
<idno type="wicri:Area/PubMed/Curation">000024</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000024</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000024</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000024</idno>
<idno type="wicri:Area/Ncbi/Merge">000452</idno>
<idno type="wicri:Area/Ncbi/Curation">000452</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000452</idno>
<idno type="wicri:Area/Main/Merge">000024</idno>
<idno type="wicri:Area/Main/Curation">000025</idno>
<idno type="wicri:Area/Main/Exploration">000025</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany.</title>
<author><name sortKey="More, G" sort="More, G" uniqKey="More G" first="G" last="Moré">G. Moré</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems, Germany. Electronic address: gastonmore@fcv.unlp.edu.ar.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems</wicri:regionArea>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Maksimov, A" sort="Maksimov, A" uniqKey="Maksimov A" first="A" last="Maksimov">A. Maksimov</name>
<affiliation wicri:level="1"><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems</wicri:regionArea>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Conraths, F J" sort="Conraths, F J" uniqKey="Conraths F" first="F J" last="Conraths">F J Conraths</name>
<affiliation wicri:level="1"><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems</wicri:regionArea>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Schares, G" sort="Schares, G" uniqKey="Schares G" first="G" last="Schares">G. Schares</name>
<affiliation wicri:level="1"><nlm:affiliation>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Greifswald, Insel Riems</wicri:regionArea>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
<wicri:noRegion>Insel Riems</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Veterinary parasitology</title>
<idno type="eISSN">1873-2550</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Coinfection</term>
<term>Foxes (parasitology)</term>
<term>Germany (epidemiology)</term>
<term>Intestine, Small (parasitology)</term>
<term>Molecular Sequence Data</term>
<term>Oocysts</term>
<term>RNA, Ribosomal, 18S (genetics)</term>
<term>Raccoon Dogs (parasitology)</term>
<term>Sarcocystis (classification)</term>
<term>Sarcocystis (genetics)</term>
<term>Sarcocystis (physiology)</term>
<term>Sarcocystosis (epidemiology)</term>
<term>Sarcocystosis (parasitology)</term>
<term>Sarcocystosis (veterinary)</term>
<term>Sequence Homology, Nucleic Acid</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Ribosomal, 18S</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Sarcocystis</term>
</keywords>
<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Germany</term>
<term>Sarcocystosis</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Sarcocystis</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Foxes</term>
<term>Intestine, Small</term>
<term>Raccoon Dogs</term>
<term>Sarcocystosis</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Sarcocystis</term>
</keywords>
<keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Sarcocystosis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Coinfection</term>
<term>Molecular Sequence Data</term>
<term>Oocysts</term>
<term>Sequence Homology, Nucleic Acid</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">More than 200 Sarcocystis spp. have been named and most of them appear to be involved in a particular predator-prey cycle. Among canids, the European fox (Vulpes vulpes) and the raccoon dog (Nyctereutes procyonoides) are widely distributed in Europe and probably play an important role as definitive hosts in the epidemiology of Sarcocystis spp. infections. A total of 50 small intestines from foxes and 38 from raccoon dogs were sampled in the Federal State of Brandenburg, Germany. Mucosal scrapings were collected and analyzed by sugar flotation and when oocysts or sporocysts were detected, an overnight sedimentation was performed and DNA extracted with a commercial kit. A PCR was conducted using primers targeting a fragment of the 18S rRNA gene (with a size of approximately 850 bp) and the amplicons were purified and sequenced. Samples with an inconclusive sequencing were cloned into plasmids and ≥ 3 plasmids from each amplicon were sequenced. Sarcocystis spp. oocysts/sporocysts were detected in 38% (19/50) of fox and 52.6% (20/38) of raccoon dog samples. Sequencing analysis of amplicons from oocyst DNA revealed mixed infections in 9 fox and 5 raccoon dog samples. In the fox samples, the most often identified Sarcocystis spp. were S. tenella or S. capracanis (10.0%); S. miescheriana (8.0%) and S. gracilis (8.0%) followed by Sarcocystis spp., which use birds as intermediate hosts (6.0%), and S. capreolicanis (4.0%). In the raccoon dog samples, sequences with a ≥99% identity with the following species were detected: S. miescheriana (18.4%), S. gracilis (13.1%), Sarcocystis spp. using birds as IH (10.5%), S. tenella or S.capracanis (2.6%) and S. capreolicanis (2.6%). The estimated prevalence of Sarcocystis spp. infections determined using mucosal scrapings was higher than in related studies performed by analyzing faecal samples. The methodology of 18S rRNA gene amplification, cloning and sequencing is suitable to identify mixed infections with Sarcocystis spp. and to gather information on potential definitive hosts of these parasite species.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
</country>
</list>
<tree><country name="Allemagne"><noRegion><name sortKey="More, G" sort="More, G" uniqKey="More G" first="G" last="Moré">G. Moré</name>
</noRegion>
<name sortKey="Conraths, F J" sort="Conraths, F J" uniqKey="Conraths F" first="F J" last="Conraths">F J Conraths</name>
<name sortKey="Maksimov, A" sort="Maksimov, A" uniqKey="Maksimov A" first="A" last="Maksimov">A. Maksimov</name>
<name sortKey="Schares, G" sort="Schares, G" uniqKey="Schares G" first="G" last="Schares">G. Schares</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/RenardV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000025 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000025 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= RenardV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:26995715 |texte= Molecular identification of Sarcocystis spp. in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Germany. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26995715" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a RenardV1
This area was generated with Dilib version V0.6.27. |